The title sequence and end credits screens are a black background with white text. However, at the end of the season 5 finale, the title is shown with black text on a white background. ("The Incident, Part 2")
Charlie put white tape on 4 fingers of his left hand onto which he wrote the letters "F A T E" with a black marker. ("Pilot, Part 1")
When Kate was preparing to sew up Jack's wound, she asked him if he had a thread color preference for the stitches, and Jack replied, "No. Standard black." ("Pilot, Part 1")
Claire sits next to Shannon on the beach as Shannon sunbathes. Claire takes off her white tank top which covered a black shirt. ("Pilot, Part 2")
Charlie and Locke sit next to each other on the log, Charlie dressed in black and Locke dressed in white. ("Tabula Rasa")
When Jack went into the fuselage to find medicine, Sawyer is there too. They discuss how much of the medicine Jack is going to use to save the US Marshall. Jack says however many it takes, and Sawyer suggests he should look at the big picture. Sawyer is wearing black, and Jack is in white. Later on in the episode, Sawyer and Jack are once again on opposing sides when it comes to saving the Marshall's life. ("Tabula Rasa")
Christian Shephard wore a black suit and white shoes. ("White Rabbit")
Jack found a leather pouch in the pocket of one of the bodies of Adam and Eve. Inside were two small square shaped polished stones; one black and one white. ("House of the Rising Sun")
Jack also put the stones back into the pouch he found them in as soon as Locke came into the cave, and they were not seen again until season six.
In Christian's office, when Jack confronted his dad about Beth's death, Jack was wearing white and Christian was wearing black. These colors also reflected Jack's 'good' and Christian's 'bad' thoughts on how to deal with the girl's death. ("All the Best Cowboys Have Daddy Issues")
Some speculate that the black and white stones (seen on Adam and Eve) were on the table in Desmond's flat. ("Flashes Before Your Eyes") However, those "stones" were likely the bottle caps from Desmond's beer bottles rather the rocks Jack found on "Adam and Eve."
The Looking Glass station was named after a Lewis Carroll book in which the main character Alice had a black mischievous cat, which was blamed for all the chaos in her dream, and a white cat, which was deemed innocent.
In Jin's flashback, he was delivering a Panda in the hospital, an animal with black and white fur. ("Ji Yeon")
In Ben's flash-forward, he confronted Charles Widmore in his bedroom where each of their faces was bisected by light. Ben's left side was illuminated in white, while his right side was dark. Widmore's was framed just the opposite of Ben's. ("The Shape of Things to Come")
As Jacob and the Man in Black talk on the beach, the Black Rock in visible in the distance as having white sails and a black body. ("The Incident, Parts 1 & 2")
In the scene when Ben was considering murdering Jacob, MIB confronted him, and Jacob spoke to him, each of their faces was bisected by light. MiB's left side was illuminated in white, while his right side was dark. Jacobs face was framed just the opposite of with the right side in light.
At the end of "The Incident, Parts 1 & 2", the Lost logo appeared as black text on a white background, as opposed to the traditional white text on a black background.
The Man In Black (In the form of John Locke) belittled Locke in the statue of Taweret; while he spoke, his face went from lighted to being in the shadows several times.("LA X, Part 1")
The spring water was black and murky instead of its usual clearness. ("LA X, Part 2")
The candidate list in the cave was written in white. ("The Substitute") The list in the lighthouse was written in black. ("Lighthouse")
During his meeting with Sayid, Keamy's face was shown with the left side brightly lit and the right side in shadows. In addition, his right eye and eyebrow were strikingly different in appearance from their left-side counterparts, giving that side of his face a demonic appearance. ("Sundown")
After, and only after Sayid killed Dogen, Sayid's right side of his face was shadowed and remained that way even into his conversation with Ben. ("Sundown")
When Dogen was talking to Sayid about the two sides (evil and good) of the scale every man has, he can be seen wearing only one black glove. So his hands were "black" and "white" while Dogan explained evil by raising his "black" hand. ("Sundown")
Scenes in 'Locke's' camp all occurred at night, while scenes on the beach with Jacob's supporters all took place in daylight. ("The Package")
There were two paintings of scales in Widmore's office, one with a black frame and the other with a white frame. In both paintings there were black rocks on one side and white rocks on the other. ("Happily Ever After") (Widmore's paintings)
Charlie's canvas shoes were all white when Desmond met him at the police station and rescued him from the water at the marina. But when he chased him down the stairs at the hospital, Charlie had black and white checkered canvas shoes. ("Happily Ever After")
When Jack came out of the forest and stared down Locke, Jack was wearing a black shirt and Locke was wearing a white shirt. ("Everybody Loves Hugo")
In the Dharma Initiative Recruiting Project, recruits were sorted at the end of the process into either of the groups "Black Swan" or "White Swan", depending on if the recruits used supplied cheats.
In November 2009, executive producer and co-creator Damon Lindelof commented on the use of the black and white theme in the backgammon scene in "Pilot, Part 2", stating: "We did know that when Locke referred to the black and the white, that ultimately that concept was going to be personified by two individuals."